Quantitation of glucosamine sulfate in plasma by HPLC-MS/MS after administration of powder for oral solution formulation

Autor: Sandro Antônio Gomes, Ricardo Rodrigues Bonfim, Enikson Pontes da Silva, Leonardo de Souza Teixeira, Ricardo Martins Duarte Byrro, Kênnia Rocha Rezende, Gerson Antônio Pianetti, Isabela Costa César, Iram Moreira Mundim, Fabiana Fernandes de Santana e Silva Cardoso, Vilmar Alves de Sousa
Rok vydání: 2011
Předmět:
Zdroj: Biomedical Chromatography. 26:851-856
ISSN: 0269-3879
DOI: 10.1002/bmc.1740
Popis: A rapid method for the quantification of glucosamine in human plasma using high-performance liquid chromatography coupled to tandem mass spectrometry was developed and validated. The sample preparation includes a simple deproteinization step, using D-(1- 13 C) glucosamine hydrochloride as an internal standard. Chromatographic separation was performed on an ACE Ciano column using isocratic elution with acetonitrile and aqueous 2mM ammonium acetate containing 0.025% formic acid (80:20). Selected reaction monitoring was performed using the transitions m/z 180.1 !m/z 72.1 and m/z 181.0 !m/z 74.6 to quantify glucosamine and internal standard, respectively. The method was validated and proved to be linear, accurate and precise over the range 50-5000ng/mL of glucosamine. Recovery rates higher than 90% were obtained for both glucosamine and internal standard. No matrix effect was detected in the samples. The validated method was successfully applied to a pharmacokinetic study after oral administration of a powder for oral solution formulation containing glucosamine sulfate. Copyright © 2011 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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