Concentration Kinetics of Secoisolariciresinol Diglucoside and its Biosynthetic Precursor Coniferin in Developing Flaxseed
| Autor: | Evangelos C. Tatsis, Christophe Hano, Ophélie Fliniaux, Albrecht Roscher, Eric Grand, Christian Paetz, Sullivan Renouard, Aina Ramsay, Bernd Schneider, François Mesnard, Jingjing Fang |
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| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Linum Kinetics Plant Science Alkaline hydrolysis (body disposal) 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Biosynthesis Drug Discovery Hplc method 2. Zero hunger Chromatography biology 010401 analytical chemistry General Medicine biology.organism_classification Secoisolariciresinol diglucoside 0104 chemical sciences Coniferin Complementary and alternative medicine chemistry Molecular Medicine Hplc dad 010606 plant biology & botany Food Science |
| Zdroj: | Phytochemical Analysis. 24:41-46 |
| ISSN: | 0958-0344 |
| Popis: | Introduction – In the plant kingdom, flaxseed (Linum usitatissimum L.) is the richest source of secoisolariciresinol diglucoside(SDG), which is of great interest because of its potential health benefits for human beings. The information about the kineticsof SDG formation during flaxseed development is rare and incomplete.Objective – In this study, a reversed-phase high-performance liquid chromatography–diode array detection (HPLC-DAD)method was developed to quantify SDG and coniferin, a key biosynthetic precursor of SDG in flaxseed.Methodology – Seeds from different developmental stages, which were scaled by days after flowering (DAF), were harvested.After alkaline hydrolysis, the validated HPLC method was applied to determine SDG and coniferin concentrations of flaxseedfrom different developing stages.Results – Coniferin was found in the entire capsule as soon asflowering started and became undetectable 20 DAF. SDGwas detected 6 DAF, and the concentration increased until maturity. On the other hand, the SDG amount in a singleflaxseed approached the maximum around 25 DAF, before desiccation started. Concentration increase between 25DAF and 35 DAF can be attributed to corresponding seed weight decrease.Conclusion – The biosynthesis of coniferin is not synchronous with that of SDG. Hence, the concentrations of SDG and con-iferin change during flaxseed development. Copyright © 2012 John Wiley & Sons, Ltd.Keywords: HPLC-DAD; coniferin; flaxseed; secoisolariciresinol diglucoside; Linum usitatissimum |
| Databáze: | OpenAIRE |
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