Methylation is an inactivating mechanism of the p16 gene in multiple myeloma associated with high plasma cell proliferation and short survival

Autor:	Marcos González, José L. Hernández, Maria Jesus Moro, Alberto Orfao, Maria V. Mateos, Maria Dolores Caballero, Julia Almeida, Ramón García-Sanz, Marta Megido, Ricardo López-Pérez, Abelardo Bárez, Enrique M. Ocio, Jesús F. San Miguel, J. Fernández-Calvo
Rok vydání:	2002
Předmět:	medicine.diagnostic_test Cell growth Hematology Methylation Cell cycle Biology medicine.disease Flow cytometry law.invention Pathogenesis law medicine Cancer research Gene Multiple myeloma Polymerase chain reaction
Zdroj:	British Journal of Haematology. 118:1034-1040
ISSN:	0007-1048
DOI:	10.1046/j.1365-2141.2002.03749.x
Popis:	Summary. In order to gain further insights into the role of the p16 gene in cell cycle regulation and the prognostic implications of its inactivation, we investigated the methylation status of the p16 gene in 98 untreated patients using a polymerase chain reaction assay based on the inability of some restriction enzymes to digest methylated sequences. Forty-one patients showed a p16 methylated gene (42%). The percentage of S-phase plasma cells (PC) in these patients was almost three times higher than in those with an unmethylated p16 gene (4·16% ± 3·37%vs 1·5% ± 1·41%, P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e5f6e6b0dbc4efc41892831ea495da50 https://doi.org/10.1046/j.1365-2141.2002.03749.x Zobrazit plný text záznamu Plný text