| Autor: |
Roger D. Zühlke, Rolf H. Joho, Hui-Juan Zhang |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Methods in Neurosciences ISBN: 9780121852955 |
| DOI: |
10.1016/s1043-9471(05)80034-5 |
| Popis: |
Publisher Summary This chapter discusses Xenopus oocytes, which is a system for expression cloning and structure–function studies of ion channels and receptors. The advantages of the Xenopus oocyte compared to other expression systems are severalfold. An individual frog egg is fairly large (0.5–1.0 mm in diameter); therefore, it can be handled with relative ease. Endogenous receptors and ion channels are mainly expressed in the layers of follicular cells surrounding the vitellin membrane of the oocyte. Because the follicular layers are removed before or after microinjection of cRNA, it is possible to express a channel or receptor of interest in denuded oocytes without signal interference of endogenously expressed gene products. The Xenopus oocyte not only possesses the necessary machinery for correct translation of foreign mRNAs but, in many instances, is capable also of carrying out posttranslational modifications that may be needed for the proper function of an oocyte-expressed protein. The unique characteristics of the oocyte expression system made it possible to express legions of foreign genes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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