Serum Assays for Detecting Pregnancy in Mule and White-Tailed Deer
| Autor: | Gary L. Dusek, Ann-Elaine Darling, Alan K. Wood, Carla A. Ruder, R. Garth Sasser, Robert E. Short |
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| Rok vydání: | 1986 |
| Předmět: |
Pregnancy
Ecology media_common.quotation_subject symbols.heraldic_supporter Zoology Radioimmunoassay Biology Odocoileus biology.organism_classification medicine.disease Blood serum Animal science symbols medicine General Earth and Planetary Sciences Gestation Domestic Cows Reproduction Ecology Evolution Behavior and Systematics Ovis canadensis Nature and Landscape Conservation General Environmental Science media_common |
| Zdroj: | The Journal of Wildlife Management. 50:684 |
| ISSN: | 0022-541X |
| Popis: | Blood samples were collected from live-trapped and manually restrained mule (Odocoileus hemionus) and white-tailed (0. virginianus) deer to evaluate the effectiveness of 2 serum tests for detecting pregnancy. Serum concentrations of progesterone and pregnancy-specific protein B (PSPB) were measured by radioimmunoassay. Both tests provided reliable means of detecting pregnancy. J. WILDL. MANAGE. 50(4):684-687 Analysis of blood serum for progesterone has been used to detect pregnancy in several wild ungulate species including white-tailed deer (Plotka et al. 1977), bighorn sheep (Ovis canadensis) (Ramsay and Sadleir 1979), and elk (Cervus elaphus) (Weber et al. 1982), but this technique apparently has not been applied to mule deer. The validity of progesterone tests for detecting pregnancy was recently questioned by Plotka et al. (1983:41) who stated that "conclusions drawn for determination of progesterone in a single blood sample can be misleading. " Analysis of blood serum for pregnancy-specific proteins provides another means of detecting pregnancy in humans (Aschheim and Zondek 1927) and horses (Cole and Hart 1942). Butler et al. (1982) isolated and partially purified PSPB from the placenta of domestic cows. A radioimmunoassay was developed for this protein and has been shown to reliably detect pregnancy in cattle approximately 24 days after breeding (Sasser et al. 1986). Measurement of a cross-reactive substance in the serum of domestic sheep showed that the PSPB assay also could be used in that species (Ruder et al. 1984). The purpose of this study was to evaluate the applicability of progesterone and PSPB assays for detecting pregnancy in free-ranging mule and white-tailed deer. We thank K. L. Hamlin, D. F. Pac, D. A. Palmisciano, and C. A. Simmons for providing blood samples; R. B. Staigmiller, W. L. Milmine, and P. M. Panlasigui for assisting in blood collection and analysis; and R. L. Kirkpartrick, R. J. Mackie, E. D. Plotka, R. B. Staigmiller, and J. P. Weigand for providing helpful comments on the manuscript. This effort received financial support from the Mont. Dep. Fish, Wildl. and Parks, Fed. Aid Proj. W-120-R; Fort Keogh Livest. and Range Res. Stn.; and the Agric. Exp. Stn., Univ. Idaho, Moscow. This paper is Mont. Agric. Exp. Stn. Article 11597. |
| Databáze: | OpenAIRE |
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