Effects of polyethyleneimine on cyanobacterium Anabaena flos-aquae during cell flocculation and flotation
| Autor: | Lu-Kwang Ju, Donald W. Ott, Sarah A. Arrington, Margo J. Zeleznik |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cyanobacteria
Flocculation Chromatography Anabaena Vesicle technology industry and agriculture Bioengineering macromolecular substances Biology biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Pigment Cytoplasm visual_art Phycocyanin visual_art.visual_art_medium Bacteria Biotechnology |
| Zdroj: | Enzyme and Microbial Technology. 32:290-293 |
| ISSN: | 0141-0229 |
| Popis: | For production of cyanobacterial gas vesicles, polyethyleneimine (PEI) was used as flocculent to enhance the collection of buoyant Anabaena flos-aquae cells by flotation. During the flocculation-flotation process, PEI was found to cause the cells to release blue pigment(s) (presumably phycocyanin) and probably other cytoplasmic materials. The kinetic profile of the pigment release was followed and compared with that of the culture flotation. The effects of PEI on the cyanobacterial cells were also examined using transmission electron microscopy. Unlike the previous reports for the effects of PEI on Gram-negative bacteria, our observations did not reveal significant morphological changes of the PEI-exposed cells of A. flos-aquae. There were no apparent negative effects on the desired product, gas vesicles, during the PEI-induced flocculation-flotation process for cell collection. |
| Databáze: | OpenAIRE |
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