Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system
| Autor: | Yukiko Yasuoka, Gou Takahashi, Saaki Tamura, Masato Ohtsuka, Hiromi Miura, Masahiro Sato |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genetically modified mouse Cerebellum General Veterinary Transgene Hippocampus Promoter General Medicine Biology urologic and male genital diseases General Biochemistry Genetics and Molecular Biology Rosa26 locus Cell biology Transgenesis 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure embryonic structures Pi medicine Animal Science and Zoology 030217 neurology & neurosurgery |
| Zdroj: | Experimental Animals. 69:287-294 |
| ISSN: | 1881-7122 1341-1357 |
| DOI: | 10.1538/expanim.20-0002 |
| Popis: | The pronuclear injection (PI)-based targeted transgenesis (PITT) method allows the generation of targeted transgenic (Tg) mice wherein a single copy of a transgene is integrated into the Rosa26 locus following PI. The Rosa26 locus allows unbiased ubiquitous expression of integrated transgenes; however, it remains little known whether tissue-specific promoters retain their functional properties when placed at the Rosa26 locus. We evaluated tissue-specific activity and reproducibility of exogenous tissue-specific promoters targeted to the Rosa26 locus by generating Thy1-Dre/Dre reporter mice using PITT and assessed spatial expression patterns of the transgenes. The Thy1 promoter targeted to the Rosa26 locus appeared active in virtually all Purkinje cells in the cerebellum and hippocampus. However, mosaic expression of the transgene under the Thy1 promoter was observed in many other organs. This phenomenon was consistent in all the Tg lines generated by PITT, indicating a high degree of reproducibility for this experiment. |
| Databáze: | OpenAIRE |
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