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Aim: Dexmedetomidine; it is widely used in anesthesia and intensive care. We aimed to examine and compare the cytotoxic, reactive oxygen species (ROS) and apoptotic effects of dexmedetomidine on kidney cells (Hek-293) in vitro at two different high and cumulative doses. Material and Methods: The half-maximum inhibitory concentration (IC50) dose of dexmedetomidine on Hek-293 cells was determined using the 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (MTT) method. Then at two different doses of the drug; apoptotic effects were determined by Annexin-V Method, morphological examinations were determined by Acridine Orange Ethidium Bromide Method and intracellular ROS levels were determined by flow cytometry. Results: The IC50 value of dexmedetomidine for Hek-293 cells was determined as 64.6559 μg/mL. Compared with the control group, doses of 50 and 100 µg/mL of dexmedetomidine tended to show cytotoxicity (p |
