| Autor: |
Igor Bryukhovetskiy, Yuri Khotimchenko, Irina Lyakhova, N. Arnotskaya, V. Shevchenko, S. Zaitsev |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Annals of Oncology. 30:xi8 |
| ISSN: |
0923-7534 |
| DOI: |
10.1093/annonc/mdz447.024 |
| Popis: |
Background Invasive growth of glioblastoma multiforme (GBM) is associated with CD133 + cancer stem cells (CSCs) and differentiated CD133- glioblastoma cells (DGCs) formed under the influence of transforming growth factor (TGF-β). The aim of the study was a comparison of CD133+ CSCs and CD133- DGCs proteomes stimulated by TGFβ. Methods The human GBM U87MG cell line was cultured with a set of growth factors to obtain CSCs. CSCs were extracted through magnetic-activated cell sorting based on the expression of 133 cluster of differentiation; CD133- DGCs were used as a control. Differentiated glioblastoma cells (CD133- DGCs) were stimulated of TGF-β1. Highperformance liquid chromatography-mass spectrometry (HPLC-MS) was used for proteome analysis. Results 589 proteins that significantly changed expression in CD133+ CSCs compared to (P Conclusion TGF-β enhances expression of the ECM-receptor interaction signaling pathway proteins in CD133- DGCs differentiated GBM cells to the level of cancer CD133+ stem cells. Legal entity responsible for the study Far Eastern Federal University. Funding Ministry of Science and Higher Education of the Russian Federation (grant no. 14.584.21.0027; ID: RFMEFI58417X0027). Disclosure All authors have declared no conflicts of interest. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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