Chronic hepatitis with double B/C infection: virological, clinical, morphological characteristics
| Autor: | Oleg I. Kiselev, Grudinin Mp, M. E. Shubina, O P Dudanova, Elpaeva Ea, Maria Pisareva, I. A. Belavina |
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| Rok vydání: | 2016 |
| Předmět: |
Hepatitis B virus
biology business.industry Hepacivirus Hepatitis C virus virus diseases Viremia General Medicine Hepatitis C Hepatitis B medicine.disease medicine.disease_cause biology.organism_classification Virology digestive system diseases 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis Coinfection Medicine 030211 gastroenterology & hepatology business Viral hepatitis |
| Zdroj: | Clinical Medicine (Russian Journal). 94:289-294 |
| ISSN: | 2412-1339 0023-2149 |
| DOI: | 10.18821/0023-2149-2016-94-4-289-294 |
| Popis: | Aim. To estimate the frequency, virological and clinical characteristics of chronic viral hepatitis (CVH) with double B/C infection. Materials and methods. We examined 282 patients with CVH. Genomes of hepatitis B virus (HBV) and hepatitis C virus (HCV) were studied by PCR in blood and liver (AmpliSens HBV and Amplisens HCV, Russia), nuclear proteins (HBcorAg HBV and NS3 HCV) were determined by immunohistochemical method (Novocastra, UK), HBV genome was sequenced by the Sanger method using ABI prism BigDye Terminator v3.1 kits and ABIPRISM 3100 analyzer (AppliedBiosystems, USA). Indices of histological activity (HAI), fibrosis, and portal vein (PV) congestion index (CI) were calculated by formula CI=SBB/LBV, where S is PV cross section area in cm2 and LBV - linear blood flow velocity in cm/s (Vivid Pro-7 apparatus, USA). Results. CVH with double B/C infection was diagnosed in 85 (30.1%) patients including 44.7% with viral genomes and proteins in the liver, 42.4% with HCV viremia, and 12.9% with HBV/HCV viremia. Maximum CVH activity was documented in patients with latent HBV/HCV viremia (ALT 157.2±59.2 U/l, HAI 11.6±1.3, fibrosis 2.8±0.7, CI 0.059±0.005); it was minimal in patients without viremia (Alt 76.25±63.0 U/l, HAI 6.7+-0.6, fibrosis 1.7±0.5, CI 0.042±0.001; p |
| Databáze: | OpenAIRE |
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