Rapid method for detecting resistance to a QoI fungicide in Plasmopara viticola populations
| Autor: | Seiichi Furuya, Hironori Kobayashi, Tsutomu Takayanagi, Seiya Saito, Shunji Suzuki |
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| Rok vydání: | 2009 |
| Předmět: |
education.field_of_study
Pesticide resistance biology Resistance (ecology) Population General Medicine biology.organism_classification Fungicide Horticulture chemistry.chemical_compound chemistry Azoxystrobin Insect Science Plasmopara viticola Strobilurin Botany Downy mildew education Agronomy and Crop Science |
| Zdroj: | Pest Management Science. 65:840-843 |
| ISSN: | 1526-498X |
| Popis: | BACKGROUND: The increasing occurrence of Qo inhibitor (QoI)-fungicide-resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide. RESULTS: The authors have developed a rapid method for detecting resistance to a QoI fungicide, azoxystrobin, in P. viticola populations using the nested PCR-RFLP method. With this method, a glycine-to-alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan. CONCLUSION: It is proposed that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for detecting azoxystrobin-resistant P. viticola populations. Copyright © 2009 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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