Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets

Autor: Paolo Somma, Matteo Massara, Raffaella Bonecchi, Achille Anselmo, Matthias Mack, Ornella Bonavita, Hilke Brühl, Massimo Locati
Rok vydání: 2016
Předmět:
DOI: 10.1016/bs.mie.2015.09.011
Popis: Chemokine receptors are differentially expressed on leukocyte subpopulations dictating their ability to migrate both in physiological and pathological conditions. Their expression is modulated during leukocyte differentiation and maturation and they can be used as markers to identify and characterize the frequency and the activation state of leukocytes present in a tissue. Here, we will describe flow cytometry approaches to detect chemokine receptors identifying subpopulations of circulating monocytes and neutrophils.
Databáze: OpenAIRE