Разработка тест-системы для анализа антител к вирусу Эпштейна–Барр методом иммуно-ПЦР
| Autor: | D. Yu. Ryazantsev, Sergey K. Zavriev, Elena V. Svirshchevskaya, M. A. Simonova, S. V. Khlgatian, Victor D. Pivovarov, T V Yegorova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Streptavidin biology Chemistry General Medicine medicine.disease_cause Virology Epstein–Barr virus Virus law.invention 03 medical and health sciences Titer chemistry.chemical_compound 030104 developmental biology Antigen law medicine biology.protein Recombinant DNA Antibody Escherichia coli |
| Zdroj: | Молекулярная биология. 52:727-734 |
| ISSN: | 0026-8984 |
| Popis: | Successful disease prevention and therapy critically depend on timely diagnosis of infections. Quantitative immuno-PCR (qiPCR) technology improves the sensitivity in the detection of antibodies to pathogens. A qiPCR-based assay was developed to determine IgG antibodies to Epstein-Barr virus (EBV) in the human blood serum. EBV nuclear protein 1 fragment (pEBV) was expressed in Escherichia coli. A synthetic single-stranded deoxyribonucleotide was conjugated to streptavidin, and the conjugate was used to detect рEBV-IgG1-biotin complexes by qiPCR. The IgG1 titers determined by qiPCR were compared to the results of enzyme-linked immunosorbent assay (ELISA). The sensitivity of qiPCR was one order of magnitude higher than that of ELISA. Thus, a highly sensitive qiPCR-based assay was developed to quantitate antibodies specific to the recombinant EBV antigen. |
| Databáze: | OpenAIRE |
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