Bio-selective surfaces by chemically amplified constructive microlithography
| Autor: | Thomas E. Mates, Edward J. Kramer, Karen E. Sohn, Joachim O. Rädler, Bert Nickel, Gerlinde Schwake, Luisa Andruzzi |
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| Rok vydání: | 2007 |
| Předmět: |
biology
Chemistry Analytical chemistry Surfaces and Interfaces Adhesion Condensed Matter Physics Octadecyltrichlorosilane Surfaces Coatings and Films chemistry.chemical_compound PEG ratio Monolayer Materials Chemistry Biophysics biology.protein Surface modification Bovine serum albumin Cell adhesion Protein adsorption |
| Zdroj: | Surface Science. 601:4984-4992 |
| ISSN: | 0039-6028 |
| DOI: | 10.1016/j.susc.2007.07.028 |
| Popis: | In this work a simple and rapid method for the creation of bio-selective surfaces is described. Specifically, electrochemical printing of an alkyl self-assembled monolayer, also known as “constructive microlithography”, was integrated with N-hydroxysuccinimide chemical group amplification to create patterns consisting of polyethyleneglycol (PEG) and octadecyltrichlorosilane (OTS) micrometer-size features. X-ray photoelectron spectroscopy (XPS) analysis was carried out near the C 1s ionization edge to characterize the patterns for the chemical groups formed in the near-surface region during the electrochemical oxidation and after functionalization. PEG patterns were then exposed to fluorescently labeled bovine serum albumin (BSA) to test their efficacy in reducing non-specific adsorption within defined areas. It was shown by fluorescence microscopy that the PEG functionalized portions of the patterns were effective in reducing the adsorption of bovine serum albumin. Finally, cell adhesion experiments were carried out on PEG patterns pre-treated with fibronectin to promote epithelial cell adhesion to non-PEGylated regions. Cell adhesion was assessed using EGFP-expressing epithelial cells and found to be highly selective for the fibronectin coated regions while maintaining healthy cell growth with gene expression. |
| Databáze: | OpenAIRE |
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