Humanes Amniontransferrin stimuliert die Progesteronproduktion von humanen Trophoblastzellen in vitro
| Autor: | Heiner Müller, D.-U. Richter, Klaus Friese, V. Briese, A. Streu, Udo Jeschke |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Amniotic fluid Cytotrophoblast business.industry Obstetrics and Gynecology Trophoblast Syncytiotrophoblasts In vitro Andrology medicine.anatomical_structure chemistry Cell culture Transferrin Placenta embryonic structures medicine business reproductive and urinary physiology |
| Zdroj: | Zentralblatt für Gynäkologie. 122:407-412 |
| ISSN: | 1438-9762 0044-4197 |
| DOI: | 10.1055/s-2000-10607 |
| Popis: | OBJECTIVE During pregnancy transferrin plays a key role as an iron transport protein to serve the increased fetal demands of iron. Transferrin is also present in relatively high concentrations in amniotic fluid [6], showing a different glycosylation compared with serum transferrin. The biological function of human amniotic fluid transferrin (hAFT) is still unknown. In addition trophoblast cells also synthesise transferrin. Transferrin synthesised by the trophoblast shows a special glycosylation. We found identical carbohydrate structure of hAFT and trophoblast transferrin. We investigated the influence of hAFT on the progesterone-, cortisol- and hCG-release of trophoblasts in culture compared with the influence of human holo- and apo-serum transferrin on the release of these hormones. MATERIAL AND METHODS Cytotrophoblast cells were prepared from human term placentae by standard trypsin-DNAse dispersion of villous tissue followed by a percoll gradient centrifugation step. When placed in culture, the trophoblasts were incubated with varying concentrations (50-300 micrograms/ml) of human amniotic fluid- and serum-transferrin. Unstimulated cells of each placenta used as controls. Culture supernatants were assayed for progesterone, hCG and cortisol by enzyme-immunometric methods. RESULTS Our results show, that the release of progesterone increased in hAFT-treated cell cultures compared to untreated cell cultures. Holo- and apo-serumtransferrin did not show any effect on the progesterone release by trophoblast cells in vitro. Neither hAFT nor holo- and apo-serum transferrin had any effect on the cortisol- and hCG-release in vitro. CONCLUSIONS Progesterone is a marker for differentiation of trophoblasts in syncytiotrophoblasts. Only hAFT stimulates the progesterone production. We suggest, that hAFT can modulate the endocrine function of trophoblast cells in culture by regulating progesterone production. |
| Databáze: | OpenAIRE |
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