Protective effect of Ruellia tuberosa L. extracts against abnormal expression of hepatic detoxification enzymes in diabetic rats
| Autor: | Yu Fang Chang, James Swi Bea Wu, Da Wei Huang, Szu Chuan Shen, Wen Chang Chang, Jou An Chen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty biology business.industry General Chemical Engineering Area under the curve General Chemistry Streptozotocin biology.organism_classification medicine.disease Superoxide dismutase 03 medical and health sciences 030104 developmental biology Endocrinology Internal medicine Diabetes mellitus Detoxification Phase II Detoxification medicine biology.protein Ruellia tuberosa business Phase I Detoxification medicine.drug |
| Zdroj: | RSC Advances. 8:21596-21605 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c8ra03321h |
| Popis: | Ruellia tuberosa L. (RTL) has been used as a folk medicine for curing diabetes mellitus in East Asia decades. This study investigated the effect of RTL on hepatic detoxification enzyme expression in diabetic rats. Male Wistar rats were fed a high fat diet (HFD) and intraperitoneally injected with streptozotocin (STZ) to induce diabetes. Subsequently, rats received oral administrations of 100 or 400 mg kg−1 body weight RTL extract, in either water (RTLW) or ethanol (RTLE), once a day for 4 weeks. The real-time PCR analyses showed that abnormality of hepatic phase I and II detoxification enzyme expression was observed in diabetic rats. However, both RTLW and RTLE significantly normalized the expression of hepatic phase I detoxification enzymes such as CYP 2E1, and expression of phase II detoxification enzymes such as UGT 1A7 and GST M1 in diabetic rats. Furthermore, we found that fasting serum glucose, hemoglobin A1C (HbA1C) and the area under the curve of oral glucose tolerance test (AUCOGTT) levels were significantly reduced in both RTLW and RTLE treated diabetic rats. Moreover, both RTLW and RTLE significantly increased the activity of hepatic anti-oxidative enzymes such as superoxide dismutase (SOD) in diabetic rats. The present study suggests that RTL may ameliorate abnormal hepatic detoxification function via alleviating hyperglycemia and enhancing hepatic antioxidant capacity in HFD/STZ-induced diabetic rats. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|