Antibody response after hepatitis B vaccine boost mapped with peptide-phage display
| Autor: | Lisbeth Ramírez Caballero, Nicolas Delaroque, Michael Szardenings |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Phage display Hepatitis B vaccine Epidemiology virus diseases Peptide Biology Applied Microbiology and Biotechnology Virology digestive system diseases 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Infectious Diseases Antibody response chemistry 030212 general & internal medicine Biotechnology |
| Zdroj: | Humboldt kolleg Ibarra 2019. |
| ISSN: | 1390-9355 1390-9347 |
| DOI: | 10.21931/rb/cs/2019.02.01.2 |
| Popis: | Recombinant hepatitis B virus vaccines confer protection by eliciting specific antibodies against the hepatitis B surface antigen (HBsAg), known as anti-HBs. However, the performance of rapid anti-HBs diagnostic tests generates concerns regarding consistency. Novel indicators of protection might be developed by monitoring changes in targeted HBsAg-epitope profile after vaccination. In this work, we test the feasibility of our peptide-phage display platform in identifying B-cell epitopes targeted at different time-points after hepatitis B vaccination. We combined this platform with a unique approach for in silico analysis of enriched sequences. Serum samples collected from one single patient who had two boosting immunizations against hepatitis B virus were used in two-rounds of selection experiments. Five epitope candidates from HBsAg were identified in silico; most of them were previously reported in the literature. Our results suggest that the number of recognized HBsAg epitopes is related to the decrease of anti-HBs over time. |
| Databáze: | OpenAIRE |
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