Efficient transformation of Marchantia polymorpha that is haploid and has very small genome DNA

Autor:	Motoyasu Honda, Chizuko Hattori, Katsuji Tani, Kenji Katoh, Masao Nasu, Taisei Shimaoka, Nobuyasu Yamaguchi
Rok vydání:	1997
Předmět:	Acetosyringone biology Agrobacterium Agrobacterium tumefaciens biology.organism_classification Applied Microbiology and Biotechnology Molecular biology Transformation (genetics) genomic DNA chemistry.chemical_compound Marchantia polymorpha Plasmid chemistry Biotechnology Southern blot
Zdroj:	Journal of Fermentation and Bioengineering. 84:519-523
ISSN:	0922-338X
DOI:	10.1016/s0922-338x(97)81904-6
Popis:	The genomic DNA content of a cultured cell of Marchantia polymorpha HYA-2F was examined using a flow cytometer. It was estimated to be 0.32 pg (C), with a G+C content of 57.1%. The DNA content was less than that of Arabidopsis thaliana. The frequency of transformation by Agrobacterium tumefaciens using a binary vector plasmid pBI121 in the presence of acetosyringone was aproximately 10%. GUS expression analysis and Southern blotting analysis of the genomic DNA of transformants revealed that all regions of T-DNA on plasmid pBI121 were integrated into the genome of M. polymorpha.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e5656601a4eaf4a6b81f690b42be6e49 https://doi.org/10.1016/s0922-338x(97)81904-6 Zobrazit plný text záznamu Full Text from ScienceDirect