Zearalenone (ZEN) detection by a single chain fragment variable (scFv) antibody
| Autor: | Xiao-Yu Du, Zong-Hua Wang, Shi-Hua Wang, Ling Lin, Yu-Mo Huang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
biology
medicine.diagnostic_test Physiology Chemistry fungi food and beverages chemical and pharmacologic phenomena General Medicine Single chain respiratory system Applied Microbiology and Biotechnology Molecular biology Horseradish peroxidase Fusion protein chemistry.chemical_compound Immunoassay biology.protein medicine Genus Fusarium Antibody Mycotoxin Zearalenone Biotechnology |
| Zdroj: | World Journal of Microbiology and Biotechnology. 24:1681-1685 |
| ISSN: | 1573-0972 0959-3993 |
| DOI: | 10.1007/s11274-008-9657-y |
| Popis: | Zearalenone (ZEN) is a mycotoxin produced by members of the genus Fusarium. ZEN detection by ELISA using single chain fragment variable (scFv) represents a novel means of mycotoxin detection and diagnosis of fungal infection. To develop immunoassay of ZEN based on scFv, ZEN was conjugated to horseradish peroxidase (HRP). ELISA and SDS-PAGE analysis showed that the covalent attachment of ZEN to HRP was successful. Anti-ZEN scFv-E-tag fusion protein was used to detect ZEN by competitive direct-ELISA (CD-ELISA) method, and the results indicated that this scFv was appropriate in ZEN detection, meaning that scFv against fungal toxin could have an application in mycotoxin detection and diagnosis of fungal infection. |
| Databáze: | OpenAIRE |
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