Association of Aberrant Promoter Methylation Changes in the Suppressor of Cytokine Signaling 3 (SOCS3) Gene with Susceptibility to Crohn's Disease
| Autor: | Golshid Sanati, Davood Jafari, Mehrdad Noruzinia, Naser Ebrahimi Daryani, Mohammad Ahmadvand, Shahram Teimourian, Nima Rezaei |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Avicenna Journal of Medical Biotechnology. |
| ISSN: | 2008-4625 2008-2835 |
| Popis: | Background: Growing evidence supports that changes in the methylation state of Inflammatory Bowel Disease (IBD)-associated genes could significantly alter levels of gene expression, potentially contributing to disease onset and progression. We supposed that alterations in DNA methylation status at promoter region within the suppressor of cytokine signaling 3 (SOCS3) gene in intestinal tissues may be involved in the susceptibility to Crohn's Disease (CD). Methods: DNA methylation status in the promoter region of the human SOCS3 gene of intestinal tissues from 15 patients with CD and 15 age- and sex-matched healthy controls were profiled using the real-time Quantitative Multiplex Methylation Specific PCR (QM-MSP) assay. Results: Based on methylation assay data profiling, we found that patients with CD showed a higher degree of methylation of the SOCS3 gene promoter region than did the healthy controls (unmethylated DNA in CD vs. healthy controls; 0.00048±0.0011 vs. 0.07±0.142, p |
| Databáze: | OpenAIRE |
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