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Date palm (Phoenix dactylifera L.) is important dioecious plant in which male and female sex flowers are on different plant. The gender identification of date palm is only possible after 4–8 years when flowers developed. The date palm fruits are produced only on female plants, therefore identification of gender in date palm plants is essential for selection of female plants at seedling stage and increasing production. Currently, there is no consistent methodology available for the sex determination in date palm. There are several DNA marker-based methods available but none can provide truthful results for date palm sex identification. Here we focused on PCR based sex determination of date palm with Glycerol-3 phosphate acetyl transferase (GPAT3) male specific primer and LOX5.1 as positive control. GPAT3 plays important role in male fertility. According to results GPAT3 amplification only occur in male date palm samples, not in female. So that, if result of PCR amplification produced two bands than male and if one band than it is female. We further validated these primers to 315 date palm samples of Kutch region of Gujarat. Out of which, from 304 samples, we can successfully identify gender of date palm with p-value of 0.809 which indicate that the technique is efficient to differentiate sex of date palm. |
