| Autor: |
Ian M Mackay, Judy A Northill |
| Rok vydání: |
2019 |
| DOI: |
10.17504/protocols.io.w6ffhbn |
| Popis: |
This protocol aims to amplify enterovirus (EV) A71 viruses but not other viruses. This protocol was designed by us. The oligonucleotides target the 5'UTR noncoding region. This is a qualitative test for investigating EV-A71 infection of humans. The test has identified both historical EV-A71 strains and contemporary strains and has confirmed these using subgenomic sequencing of partial 5'UTR-VP2 and partial VP1 sequencing. Culture of the virus is not required as this assay is capable of detecting EV-A71 direct from extracted clinical samples. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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