Quantitative determination of the MDR-related P-glycoprotein, Pgp 170, by a rapid flow cytometric technique
| Autor: | Madeleine Bourdeaux, Félix A. Montero Julian, Michele Chauvet, Valérie L. Ferrand, Michel H. Hirn |
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| Rok vydání: | 1996 |
| Předmět: |
endocrine system diseases
integumentary system biology medicine.diagnostic_test medicine.drug_class Cell Biophysics Cell Biology Hematology Monoclonal antibody Molecular biology female genital diseases and pregnancy complications Epitope Quantitative determination Pathology and Forensic Medicine Flow cytometry carbohydrates (lipids) Multiple drug resistance Endocrinology medicine.anatomical_structure polycyclic compounds biology.protein medicine Integral membrane protein P-glycoprotein |
| Zdroj: | Cytometry. 23:120-125 |
| ISSN: | 1097-0320 0196-4763 |
| DOI: | 10.1002/(sici)1097-0320(19960201)23:2<120::aid-cyto5>3.0.co;2-o |
| Popis: | Pgp 170 is the main integral membrane protein involved in acquired or de novo multidrug resistance (MDR), frequently implicated in chemotherapeutic failure. Because there is at present no method for quantitating Pgp 170 levels, a new and convenient assay, using flow cytometry with a standard fluorescence curve and MRK 16, a mAb recognizing an external epitope of human Pgp 170, was developed. Assuming a 1:1 stoichiometry, we calculated for the first time the apparent number of Pgp 170 molecules per cell. The method was applied successfully to cells in suspension or grown as monolayers and their mixtures. All quality criteria were checked and proved the suitability of the method for quantifying Pgp 170. © 1996 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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