Monogalactosyldiacylglycerol Confers Fusogenicity to Liposomal Delivery Systems and Facilitates Targeting to Hepatocytes

Autor:	D. J. Wiseman, Murray S. Webb, B. Brett Finlay, Carol Cordeiro
Rok vydání:	1999
Předmět:	Cell specific Liposome Galactolipid media_common.quotation_subject Cell Pharmaceutical Science Lipid bilayer fusion Fusogenic liposome Biology Cell biology medicine.anatomical_structure Membrane Biochemistry medicine Internalization media_common
Zdroj:	Journal of Liposome Research. 9:461-475
ISSN:	1532-2394 0898-2104
DOI:	10.3109/08982109909035547
Popis:	Fusogenic liposomes typically rely on unsaturated phosphatidyleth-anolamines, usually dioleoylphosphatidylethanolamine (DOPE), to confer the ability to fuse with cell membranes and deliver drug intracellularly. Targeted fusogenic liposomes require an additional component to facilitate binding to, and internalization within, specific cell populations. This report examines the ability of the unsaturated galactolipid monogalactosyldiacylglycerol (MGDG) to replace DOPE in pH-sensitive fusogenic liposomes and to simultaneously confer enhanced binding to hepatocytes. Liposomes prepared using the pH-sensitive lipid N-succinylDOPE and either DOPE, MGDG or a non-fusogenic control galactolipid were compared for (i) propensity to undergo either pH-or calcium-induced membrane fusion; (ii) ability to deliver encapsulated gen-tamicin to infections of Salmonella typhimurium in murine macrophages; and (iii) binding and uptake into human hepatoma cells. Liposomes composed of MGDG/N-succinylDOPE were very similar t...
Databáze:	OpenAIRE
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