Fiber optic particle plasmon resonance immunosensor for rapid and sensitive detection of methamphetamine based on competitive inhibition
| Autor: | Chang-Yue Chiang, Lai-Kwan Chau, Day-Shin Hsu, Shau-Chun Wang, Chin-Jung Kuo, Ting-Chou Chang, I-Kuang Chen, Szu-Shan Shieh, Yung-fou Chen, Min-Hsiu Lin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Optical fiber Materials science Chromatography biology 010401 analytical chemistry Nanoparticle 02 engineering and technology Buffer solution 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry law.invention chemistry.chemical_compound chemistry law biology.protein Fiber Bovine serum albumin Surface plasmon resonance 0210 nano-technology Biosensor Spectroscopy |
| Zdroj: | Microchemical Journal. 157:105026 |
| ISSN: | 0026-265X |
| Popis: | We report the study of methamphetamine (MA) sensing in urine samples using fiber optic particle plasmon resonance (FOPPR) biosensor. This approach is intended for on-site analysis of low-molecular-weight compounds. High sensitivity detection of MA at ultra-low concentration is realized by using a competitive inhibition immunoreaction scheme based on the competition of free MA in solution for anti-MA molecules that bind to the bovine serum albumin (BSA)–MA conjugate on the gold nanoparticle surface. With the BSA–MA functionalized sensor fiber, in the presence of a fixed concentration of anti-MA and various concentrations of MA in sample, the change of transmitted light intensity through the sensor fiber relative to that in a buffer solution decreases when the MA concentration increases because of the inhibition effect of MA. Based on this sensing method, the MA-functionalized FOPPR biosensor is capable for determining the concentration of MA with high sensitivity and wide linear dynamic range of 1–1000 ng/mL. The limit of detection for MA is 0.16 ng/mL. In addition, the MA functionalized FOPPR biosensor can detect MA in diluted human urine samples without nonspecific adsorption interference. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect