The Use of Thermotherapy andin vitroMeristem Culture to Produce Virus-Free ‘Chancellor’ Grapevines
| Autor: | M. A. Norton, Richard Mulwa, Robert M. Skirvin, Mark E. Uchanski, R. A. Martĩnez-Peniche, A. J. Dĩaz-Barrita |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | International Journal of Fruit Science. 7:15-25 |
| ISSN: | 1553-8621 1553-8362 |
| DOI: | 10.1300/j492v07n03_03 |
| Popis: | Grapevines (Vitisspp.) are very susceptible to virus diseases. Virus infection reduces fruit yield and quality. The objective of this work was to determine the usefulness of thermotherapy (37.2°C) and in vitromeristem culture to obtain virus-free grapevine plants cv. ‘Chancellor’. Grapevine leafroll-associated virus 1,3(GLRaV-1, 3) infected grapevines were multiplied in vitrofrom two infected mother-plants in half strength Murashige and Skoog medium (1/2MS) supplemented with 0.5 mg/L of BA and the in vitroplants were initially tested by ELISA to confirm their virus status; subsequently, 96 infected in vitroplants were propagated on 1/2MS medium with BA and subjected to 0, 7, 10, 12, 14, 16, 18, 20 or 22 days of exposure at 37.2°C. Afterward, the apical meristems from the plants surviving the thermotherapy treatment were excised and transferred to fresh 1/2MS medium with 0.5 mg/L of BA and grown in a culture room until they developed into entire plants. Control plants and all the plants that survi... |
| Databáze: | OpenAIRE |
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