Autor:	Paul Kiefer, B. Wegmann, Marianne Antoine
Rok vydání:	1998
Předmět:	Cloning biology Cytomegalovirus General Medicine biology.organism_classification medicine.disease_cause Virology Epithelium Long terminal repeat respiratory tract diseases Retrovirus medicine.anatomical_structure Cell culture Murine leukemia virus Genetics medicine Molecular Biology Gene
Zdroj:	Virus Genes. 17:157-168
ISSN:	0920-8569
Popis:	Development of methods for gene transfer into specific cell types or tissues is important for experimental research as well as clinical therapeutical approaches. We report here the cloning and characterization of the envelope (env) gene and the U3 region of a retrovirus from an infected human Small Cell Lung Cancer (SCLC) cell line. The replication of this murine retrovirus is also fully supported by other lung cancer cell lines of different histological origin. We present evidence that a long terminal repeat (LTR)-β-galactosidase (β-Gal) reporter construct performed as well as an analogous cytomegalovirus (CMV) promoter β-Gal construct in the human lung epithelial cell line A549 and in the human larynx carcinoma cell line HEp2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e525d0d3aecea45bcff3325fcdd75993 https://doi.org/10.1023/a:1008020808314 Zobrazit plný text záznamu Full text from SpringerLink