| Autor: |
Grigor V. Gyulkhandanyan, Anichka S. Hovsepyan, Marina H. Paronyan, Artak Tovmasyan, Aram G. Gyulkhandanyan, Gayane V. Amelyan, Robert K. Ghazaryan, Anna G. Gyulkhandanyan |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
SPIE Proceedings. |
| ISSN: |
0277-786X |
| Popis: |
Photodynamic inactivation (PDI) of microorganisms is successfully applied against Gram (+) microorganisms. However the majority of photosensitizers poorly affect on Gram (-) microorganisms. At present number of works have shown that cationic photosensitizers are able to induce photoinactivation both Gram (+) and Gram (-) microorganisms. The purpose of this work was definition of more effective new cationic pyridylporphyrins with various coordinated metals and functional groups for destruction of Gram (-) microorganisms. The efficiency of new cationic porphyrins and metalloporphyrins (9 compounds) was tested against Gram (-) microorganism E. coli (strain Κ-12). The testing results show high efficiency of metalloporphyrins, especially silver complexes, against E. coli microorganism under dark conditions. 50 % and 100 % cell growth inhibitory concentrations (IC50 and IC100 values, accordingly) of studied metallocomplexes are considerably lower in comparison with metal-free porphyrins. At the same time the Zncomplexes of porphyrins are more phototoxic than their metal-free analogues. Zn-metalloporphyrins with allyl and butyl functional groups were shown the highest efficiency against E. coli. The photodynamic action of cationic Zn -TBut4PyP metalloporphyrin against Gram(+) (St. aureus and St. epidermis) and Gram(-) (E.coli, strain K-12 and Salmonella sp.) microorganisms was investigated. It is revealed, that Gram (+) microorganisms were 3-5 times more susceptible to the compounds' phototoxic influence than Gram (-) microorganisms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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