The Effect of Culture Physical Conditions and Explant Type and Source on the Growth and Multiplication of Ficus benjamina in Vitro
Autor: | P.G. Alderson, N.S. Al-Khalifah |
---|---|
Rok vydání: | 1995 |
Předmět: | |
Zdroj: | HortScience. 30:873F-873 |
ISSN: | 2327-9834 0018-5345 |
DOI: | 10.21273/hortsci.30.4.873f |
Popis: | Shoot cultures of Ficus benjamina cv. Cleo showed a variable response to light intensity in the range of 8 to 50 μmol–m–2–s–1. The highest leaf area occurred at 15 μmol–m–2–s–1 and shoot length and chlorophyll contents increased with the decrease of light intensity. Incubating explants for 8 weeks at 25 and 35C showed that shoot length increased at 35C; however, shoot multiplication, leaf number, root number, and callus weight were higher at 25C. Shoot-tip explants were superior to axillary buds when cultured or subcultured for shoot elongation, whereas axillary buds were better for shoot multiplication. Shoot tips with two or three small leaves appeared to be the best explants for fast growth and healthy cultures of F. benjamina. |
Databáze: | OpenAIRE |
Externí odkaz: |