Concomitant yield optimization of tannase and gallic acid by Bacillus licheniformis KBR6 through submerged fermentation: An industrial approach
| Autor: | Pradeep K. Das Mohapatra, Keshab Chandra Mondal, Bikas R. Pati, Ishita Biswas |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Chemistry Fructose Phosphate biology.organism_classification 01 natural sciences General Biochemistry Genetics and Molecular Biology Tannase 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Yield (chemistry) Tannic acid Urea Gallic acid Bacillus licheniformis Food science General Agricultural and Biological Sciences 030304 developmental biology |
| Zdroj: | Acta Biologica Szegediensis. 64:151-158 |
| ISSN: | 1588-4082 1588-385X |
| DOI: | 10.14232/abs.2020.2.151-158 |
| Popis: | The present study is concerned with the evaluation of tannase and gallic acid production effi cacy of Bacillus licheniformis KBR6 under diff erent environmental conditions through submerged fermentation. Results have shown that diff erent environmental conditions and mineral sources have diff erential infl uences on tannase and gallic acid production. Highest tannase and gallic acid yield was observed at incubation period of 18 h and 22 h, respectively. At tannic acid concentration of 15 g/l, maximum cell mass (0.75 g/l), cell yield coeffi cient (0.08 g/g), specifi c growth rate (37.5 mg/g/h), tannase yield (16.3 U/g) and specifi c tannase production rate (0.80 U/g/h) were observed, however, at higher tannic acid concentration a decrease in tannase yield and production rate were observed, but gallic acid production increased with increasing tannic acid concentration. Additional carbohydrate sources like glucose, fructose, and lactose showed positive infl uence on enzyme yield. Among the studied nitrogen sources urea and NH4Cl, and of the phosphate sources KH2PO4 showed favourable eff ects on cell growth and simultaneous enzyme and gallic acid production. Temperature of 35 °C was found to be optimum for tannase and gallic acid production. Of all the studied metal ions Ca2+, Mg2+ and Na+ showed positive eff ect whereas, Co2+, Ag2+, Pb2+, Hg2+ showed inhibitory eff ects. |
| Databáze: | OpenAIRE |
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