Oral rinse MMP-8 point-of-care immuno test identifies patients with strong periodontal inflammatory burden
| Autor: | Marcela Hernández, V-J Uitto, L. Netuschil, Päivi Mäntylä, M-M Ahonen, Timo Sorsa, Jussi Leppilahti, S. K. Munjal |
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| Rok vydání: | 2010 |
| Předmět: |
Doxycycline
0303 health sciences medicine.medical_specialty Receiver operating characteristic Gingival and periodontal pocket business.industry Bleeding on probing Case-control study Dentistry 030206 dentistry Matrix metalloproteinase Gastroenterology 3. Good health 03 medical and health sciences 0302 clinical medicine Otorhinolaryngology Internal medicine Severity of illness Medicine Biomarker (medicine) medicine.symptom business General Dentistry 030304 developmental biology medicine.drug |
| Zdroj: | Oral Diseases. 17:115-122 |
| ISSN: | 1354-523X |
| Popis: | OBJECTIVE: To determine whether oral rinse matrix metalloproteinase (MMP)-8 levels, measured by three different methods, tissue inhibitor of matrix metalloprotease-1 (TIMP-1) levels and elastase activity differentiate subjects with different periodontal condition; and second, to find out if MMP-8 levels were comparable among the methods used. METHODS: MMP-8 levels were analysed with an immunofluorometric method (IFMA), dentoELISA and commercial ELISA. Also TIMP-1 levels and elastase activity were measured. For statistical analysis 214 study subjects were categorized into four groups, specified by the presence and number of moderate (4‐5 mm) and deep (‡6 mm) periodontal pockets, and bleeding on probing percentage. RESULTS: MMP-8 levels especially measured by dentoELISA and adjusted to the number of teeth per subject differentiated the study group with strong periodontal inflammatory burden from groups with lower levels. This was also verified with receiver operating characteristic (ROC) analysis. Elastase activity associated with higher IFMA and dentoELISA MMP-8 levels. IFMA MMP-8 ⁄TIMP and dentoELISA MMP-8 ⁄TIMP-1 tended to be higher with the increasing level of periodontal inflammatory burden. TIMP-1 levels decreased with increasing age. CONCLUSIONS: Oral rinse MMP-8 together with TIMP-1 analysis may have potential in complementary periodontal diagnostics. dentoELISA can be applied in quantitative oral rinse chair side biomarker diagnostics. Oral Diseases (2011) 17, 115–122 |
| Databáze: | OpenAIRE |
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