Polyploidy: a missing link in the conversation about seed transfer of a commonly seeded native grass in western North America
| Autor: | Lila Fishman, Cara R. Nelson, Alexis L. Gibson |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
education.field_of_study Ecology biology Tussock fungi Population food and beverages Native plant biology.organism_classification Fecundity 010603 evolutionary biology 01 natural sciences Polyploid Botany Pseudoroegneria spicata Revegetation Ploidy education Ecology Evolution Behavior and Systematics 010606 plant biology & botany Nature and Landscape Conservation |
| Zdroj: | Restoration Ecology. 25:184-190 |
| ISSN: | 1061-2971 |
| Popis: | The use of local, native plant materials is now common in restoration but testing for polyploidy in seed sources is not. Diversity in cytotypes across a landscape can pose special seed transfer challenges, because the methods used to determine genetically appropriate materials for seed transfer do not account for cytotypic variation. This lack of consideration may result in mixing cytotypes through revegetation, which could reduce long-term population viability. We surveyed nine populations of a native bunchgrass, Pseudoroegneria spicata, in three EPA Level III Ecoregions in the western United States to determine the frequency of polyploidy, whether there are differences in traits (phenotype, fecundity, and mortality) among plants of different cytotypes, and whether cytotype frequency varies among ecoregions. We assessed trait variation over 2 years in a common garden and determined ploidy using flow cytometry. Polyploidy and mixed cytotype populations were common, and polyploids occurred in all ecoregions. Four of the nine populations were diploid. The other five had tetraploids present: three had only tetraploid individuals whereas two had mixed diploid/tetraploid cytotypes. There was significant variation in traits among cytotypes: plants from tetraploid populations were larger than diploid or mixed populations. The frequency and distribution of cytotypes make it likely that seed transfer in the study area will inadvertently mix diploid and polyploid cytotypes in this species. The increasing availability of flow cytometry may allow ploidy to be incorporated into native plant materials sourcing and seed transfer. |
| Databáze: | OpenAIRE |
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