| Autor: |
Riksen T, Dehe Cheng, Zhang J, Sanwen Huang, Yawen Wang, Kim Boutilier, Yu Zhong, Mengdong Wang, Zhenshan Liu, Minhui Chen, Jianfeng Liu, Chenxu Liu, Wenjie Liu, Baojian Chen, Yanyan Jiao, Yanlong Li, Xiaolong Qi, Chen Chen, Zhu X, Bin Yi, Jinyun Li, Mingna Li, Dan Wang, Shanshan Chen |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.1101/2021.06.21.449224 |
| Popis: |
Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, which significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is been widely and efficiently used in maize and was recently extended to several monocot crops. However, a similar generic and efficient HI system is still lacking in dicot crops. Here we show that genotype-independent in vivo HI can be triggered by mutation of DMP genes in tomato, rapeseed and tobacco with HI rates of ~1.9%, 2.4% and 1.2%, respectively. The DMP-HI system offers a robust DH technology to facilitate variety improvement in these crops. The success of this approach and the conservation of DMP genes paves the way for a generic and efficient genotype-independent HI system in other dicot crops. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
