Generation of bright monomeric red fluorescent proteins via computational design of enhanced chromophore packing
| Autor: | Sandrine Legault, Derek P. Fraser-Halberg, Ralph L. McAnelly, Matthew G. Eason, Michael C. Thompson, Roberto A. Chica |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Chemical Science. 13:1408-1418 |
| ISSN: | 2041-6539 2041-6520 |
| DOI: | 10.1039/d1sc05088e |
| Popis: | We used computational design to increase quantum yield in a fluorescent protein by optimizing chromophore packing to reduce non-radiative decay, resulting in an >10-fold increase in quantum yield that was further improved by directed evolution. |
| Databáze: | OpenAIRE |
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