Binding of the Host–Guest Complex of 7-Aminoflavone/β-Cyclodextrin with Calf Thymus DNA: A Spectroscopic and Molecular Docking Study

Autor:	V. Santhanam, Israel V.M.V. Enoch, Sowrirajan Chandrasekaran, Y. Sameena
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Cyclodextrin Molecular model Stereochemistry Biophysics Biochemistry Binding constant Fluorescence chemistry.chemical_compound chemistry Excited state Molecule Physical and Theoretical Chemistry Absorption (chemistry) Molecular Biology DNA
Zdroj:	Journal of Solution Chemistry. 43:1132-1146
ISSN:	1572-8927 0095-9782
DOI:	10.1007/s10953-014-0187-y
Popis:	The selective binding of 7-aminoflavone (7-AF) molecules with DNA was investigated in the host–guest complex form with β-cyclodextrin (β-CD). The host–guest association of 7-AF with β-CD was investigated using absorption, steady-state fluorescence, time-resolved fluorescence and NMR spectroscopic techniques. The stoichiometry and the binding constant of the inclusion complex were determined. The possible orientation of the 7-AF molecule to the β-CD was confirmed by the 2D ROESY NMR technique and was further studied by molecular modeling. The ground and the excited state pK a values are reported for the free guest molecule and the β-CD complex. The binding of 7-AF to calf thymus DNA with and without the β-CD are reported. The binding constant of DNA with 7-aminoflavone in the presence and the absence of β-CD were calculated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e4bb1e6825a98aa975e28eea9f9e4f14 https://doi.org/10.1007/s10953-014-0187-y Zobrazit plný text záznamu Full text from SpringerLink