Studies on neurosteroids XVIII
| Autor: | Yasuaki Ninomiya, Tatsuya Higashi, Naoki Takayama, Akinori Yamauchi, Kazutake Shimada, Naoko Iwaki |
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| Rok vydání: | 2006 |
| Předmět: |
Pharmacology
medicine.medical_specialty Neuroactive steroid medicine.drug_class Chemistry Metabolite Organic Chemistry Clinical Biochemistry Endogeny Androgen Biochemistry Anxiolytic chemistry.chemical_compound Endocrinology Internal medicine medicine Animal studies Receptor Molecular Biology Testosterone |
| Zdroj: | Steroids. 71:609-617 |
| ISSN: | 0039-128X |
| Popis: | The liquid chromatography-mass spectrometry (LC-MS) methods were developed and validated for the determination of testosterone (T) in the brain and serum of rats and of 5alpha-androstane-3alpha,17beta-diol (ADIOL), a metabolite of T, in the brain of rats. After derivatization of T with 2-hydrazino-1-methylpyridine and of ADIOL with p-nitrobenzoyl chloride, the detection sensitivities of T and ADIOL using LC-MS were increased 70- and 400-times superior to those of intact T and intact ADIOL, respectively. Those LC-MS methods are specific and reliable for the analysis of trace amounts of T and ADIOL in small amounts of samples. The animal studies using the developed methods showed that the brain and serum levels of T and the brain levels of ADIOL were not changed by stress or ethanol administration but the concentration ratio of the brain T to serum T in the stressed rats was higher than that in untreated rats. The low levels of endogenous AIDOL in brain of stressed and unrestrained rats found in this study demonstrated that the contribution to anesthetic and anxiolytic effects of ADIOL via gamma-aminobutyric acid type A receptors may be negligible. |
| Databáze: | OpenAIRE |
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