Effect of Artemisia absinthium ethanolic extract on oxidative stress markers and the TLR4, S100A4, Bax and Bcl-2 genes expression in the kidney of STZ-induced diabetic rats
| Autor: | Mehdi Salehi, Jamal Amri, Seied Amirhossein Latifi, Abbas Alimoradian, Fatemeh Bagheri, Hadi Karami |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Pharmacology medicine.disease_cause Absinthium Diabetic nephropathy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Diabetes mellitus medicine Molecular Biology Kidney Creatinine biology medicine.diagnostic_test business.industry General Medicine medicine.disease biology.organism_classification 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Artemisia absinthium Lipid profile business Oxidative stress |
| Zdroj: | Hormone Molecular Biology and Clinical Investigation. 41 |
| ISSN: | 1868-1891 1868-1883 |
| Popis: | Objectives The present study was conducted to examine antidiabetic effects of Artemisia absinthium ethanolic extract [A. absinthium] and to investigate its effects on oxidative stress markers and the expression of TLR4, S100A4, Bax and Bcl-2 genes in the kidney of STZ-induced diabetic rats. Methods Thirty six rats (weight 200–250 g) were randomly divided into diabetes and control groups. Induction of diabetes was performed using STZ (55 mg/kg.bw). Biochemical parameters and oxidative stress markers (SOD and MDA) were measured using spectrophotometry after 60 days of treatment. The expression of TLR4, S100A4, Bax and Bcl-2 were analyzed by real-time PCR. One-way analysis of variance (ANOVA) and Bonferroni post hoc test were used to compare the data. Results Diabetes significantly impairs the serum fasting blood glucose (FBG), lipid profile, urea, creatinine and albumin. At the end of treatment with A. absinthium extract, these parameters were close to the normal range. The results showed that the A. absinthium extract significantly decreased the kidney expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress markers (SOD and MDA) in the kidney tissues of treated rats. Also, all of these beneficial effects of the A. absinthium were dose-dependent. Conclusions The extract of A. absinthium possesses antidiabetic effects. A. absinthium decreased the expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress. Therefore, this herbal extract can be used as an adjuvant treatment for diabetic complications. |
| Databáze: | OpenAIRE |
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