High yellow pigments production by root culture of Carthamus tinctorius and its release in medium under gas oil treatment
| Autor: | Françoise Bernard, Zahede Chaghari, Sahar Hassannejad, Azar Hassanpour, Gila Gholizadeh |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chromatography
biology medicine.diagnostic_test Physiology Chemistry Carthamus Plant physiology Plant Science Fuel oil Liquid medium biology.organism_classification Pigment Horticulture Volume (thermodynamics) Spectrophotometry visual_art medicine visual_art.visual_art_medium biology.protein Agronomy and Crop Science Peroxidase |
| Zdroj: | Acta Physiologiae Plantarum. 33:431-436 |
| ISSN: | 1861-1664 0137-5881 |
| DOI: | 10.1007/s11738-010-0562-2 |
| Popis: | In this study, the ability of safflower-isolated root cultures to produce yellow pigments was tested. Initially, the growth of isolated roots in static liquid medium was evaluated with different volumes of culture medium. A volume of 6 ml of medium per flask of 250 ml gave the best growth performance and, in this condition of culture, production of pigments from isolated roots treated or not by light has been determined by spectrophotometry (321 and 400 nm). Under these conditions, the production of yellow pigments amounted to 13.18 mg g−1 fresh weight and the light stimulated the synthesis of these pigments by isolated roots. Total yellow pigments of 24.12, 38.91 and 46.38 mg g−1 fresh weight was produced by the roots treated with 9, 13.5 and 18% (v/v) gas oil, respectively, representing high values of production. The pigments were released in large quantities in the medium. The increased synthesis of pigments as a result of gas oil treatment was accompanied by a reduction of the peroxidase activity of roots. Given the high production of yellow pigments, systems of isolated root culture could be considered for the study of a larger scale production of safflower pigments widely used for various industrial purposes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink