Optimization of roller culturing of influenza virus vaccine strains in MDCK and Vero cell cultures using plant origin components

Autor:	G. P. Troshkova, Larisa N. Shishkina, Skarnovich Mo, N. A. Mazurkova, E. I. Isaeva, R. Ya. Podchernyayeva
Rok vydání:	2011
Předmět:	Bromelain (pharmacology) Influenza vaccine Biology Trypsin Cell morphology Applied Microbiology and Biotechnology Biochemistry Virology Hydrolysate Microbiology Titer Vero cell medicine Influenza virus vaccine medicine.drug
Zdroj:	Applied Biochemistry and Microbiology. 47:737-743
ISSN:	1608-3024 0003-6838
Popis:	Roller culturing of MDCK and Vero cells in an experimental nutrient medium based on soy flour hydrolysate, plant material obtained using the bromelain plant enzyme, was studied. The medium supplemented with 2 or 3% fetal calf serum (FCS) had a strong growth-stimulating effect on Vero and MDCK and cells, respectively, and did not alter the cell morphology. A/Solomon Islands/03/06 (H1N1) and B/Malaysia/2506/04 influenza vaccine viruses were grown on MDCK and Vero cell cultures obtained as a result of culturing in rollers on media containing soy flour hydrolysate and FCS (2 or 3%, respectively). The titer of the viruses was high in the presence of either trypsin (2 μg/ml) or bromelain (20 μg/ml).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e4811c5f3dc50fdb934195b78f5206fd https://doi.org/10.1134/s0003683811080059 Zobrazit plný text záznamu Plný text ve formátu PDF