Phenotypical changes in hop induced by micropropagation

Autor: Rúbia de Souza, Jefferson Luís Meirelles Coimbra, Adriano Michel, Altamir Frederico Guidolin, Cristiane Rosa Adams, Rita Carolina de Melo
Rok vydání: 2021
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture (PCTOC). 147:379-387
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-021-02130-2
Popis: Micropropagation is a promising technique for hop (Humulus lupulus L.) plantlet production. However, the phenotypic stability of micropropagated hop plants is poorly understood. These should be characterized, in order to identify possible variations. The purpose of this study was to analyze and characterize the occurrence of somaclonal variation induced by in vitro multiplication of hop genotypes. Three genotypes were established in vitro and micropropagated in five subcultures. The response variables were chosen by the stepwise method and subjected to multivariate analysis of variance (Manova), multivariate contrasts, standardized canonical coefficients (StCC) and intraclass correlation (t). Somaclonal variation was detected in the genotypes Chinook and Columbus, both from America. No significant variations were found in the European genotype Hallertauer Mittelfrueh, regardless of the number of subcultures. Somaclonal variation was detected in the genotypes Chinook and Columbus, both from America. No significant variations were found in the genotype Hallertauer Mitterlfrueh, regardless of the number of subcultures.
Databáze: OpenAIRE