Structure and kinetics of synthetic, lipid-based nucleic acid carriers
| Autor: | Nily Dan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Kinetics Cationic polymerization Gene Abnormality 02 engineering and technology 021001 nanoscience & nanotechnology In vitro 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure chemistry In vivo medicine Nucleic acid Biophysics lipids (amino acids peptides and proteins) 0210 nano-technology DNA |
| DOI: | 10.1016/b978-0-12-813687-4.00014-1 |
| Popis: | Nucleic acid–based therapies use DNA or siRNA to treat genetic disorders caused by gene abnormality, such as hemoglobinopathies (sickle cell disease, beta-thalassemia), cystic fibrosis, or some types of cancer. Complexes between nucleic acids and cationic phospholipids, lipoplexes, are a promising, synthetic gene carrier for both in vitro and in vivo applications. However, optimization of the complex performance requires better understanding and control of lipoplex properties. Lipoplex structure is characterized on two lengths. On the local scale, namely, 10–50 nm, the lipid and nucleic acid arrangement is set by thermodynamic equilibrium and is directly linked to the molecular properties of the lipids and concentration of nucleic acid. On larger scales, in the order 100 nm–1 μm, lipoplex characteristics are set by the kinetics of the synthesis process, following a three-step process: (1) an initial stage (on timescales of less than 1 min) where the nucleic acid adsorbs onto the lipid assembly; (2) a growth stage, where complexes flocculate, characterized by a broad range of timescales from minutes to hours, depending on system parameters; and (3) internal equilibration stage where the outer lipoplex structure remains constant, which may take hours or more. |
| Databáze: | OpenAIRE |
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