Electrospun polycaprolactone/silk fibroin nanofibrous bioactive scaffolds for tissue engineering applications
| Autor: | Emel Yilgor, Muhammad Anwaar Nazeer, Iskender Yilgor |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Aqueous solution
Polymers and Plastics biology Chemistry Formic acid fungi Organic Chemistry technology industry and agriculture Fibroin 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences 0104 chemical sciences Solvent chemistry.chemical_compound SILK Tissue engineering Chemical engineering Bombyx mori Polycaprolactone Materials Chemistry 0210 nano-technology |
| Zdroj: | Polymer. 168:86-94 |
| ISSN: | 0032-3861 |
| Popis: | Degumming of Bombyx mori silk cocoons by a novel and mild process using aqueous ammonia and fabrication of electrospun polycaprolactone/silk fibroin (PCL/SF) nanofibrous scaffolds is reported. Cocoons were degummed in 0.3% w/w solutions of boiling ammonia (28–30%) for 45 min. Degummed SF fibers were dissolved in phosphoric and formic acid (7/3 v/v) mixture, coagulated in methanol, filtered and dried. PCL solutions containing different amounts of SF were electrospun in formic acid, a green solvent. Scaffolds were characterized to confirm the successful incorporation of SF and to demonstrate formation of nanofibrous webs with good biomechanical properties. Cell viability assay was performed by seeding Human BJ fibroblast cells on scaffolds. In vitro analysis showed that the scaffolds produced were non-toxic and incorporation of SF resulted in enhanced cell proliferation. Nanofibrous PCL/SF scaffolds with good biomechanical properties developed through dialysis free processing of silk fibroin can be promising substrates for tissue engineering applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect