Genotyping-by-sequencing to determine the genetic structure of a Tibetan medicinal plant Swertia mussotii Franch

Autor: Yi Wang, Yong Q. Gu, Yue Liu, Xixi Zuo, Jiaqing Sun, Yan Qiao, Zeguang Guo, Lin Zhan, Naxin Huo, Fengxian Guo
Rok vydání: 2020
Předmět:
Zdroj: Genetic Resources and Crop Evolution. 68:469-484
ISSN: 1573-5109
0925-9864
Popis: Swertia mussotii Franch. has been widely used in Tibetan folk medicine as an important drug ingredient for the treatment of hepatitis and hepatobiliary disease and for homeopathic purposes. But the lacking natural resources of S. mussotii is becoming more obvious with increasing market demands. The main objective of this work was to examine genetic differentiation among S. mussotii populations from different geographical locations and use the knowledge gained to facilitate future selection of the species suitable for large-scale cultivation to meet the increasing demand for medicinal use of this plant. In this study, 68 S. mussotii Franch samples collected from Zhongda (16), Chengduo (16), and Xiewu (8) County in Qinghai Province, Xiaojin (14) and Jinchuan (14) County in Sichuan Province, China and 4 samples from related species, were genotyped using genotyping-by-sequencing (GBS) technique. GBS scored 4095 SNPs with a minor-allele frequency > 0.25 and an average read depth
Databáze: OpenAIRE