Isolation and identification of locally isolated bacterial strains effective against whiteflyBemisia tabaci

Autor:	Salah El-Din El-Assal, G. Amin, Narmen A. Youssef
Rok vydání:	2013
Předmět:	Veterinary medicine Soil Science Whitefly Biology 16S ribosomal RNA Pathogenicity biology.organism_classification Isolation (microbiology) DNA sequencing RAPD Botany Instar Nymph Agronomy and Crop Science
Zdroj:	Archives of Agronomy and Soil Science. 59:779-790
ISSN:	1476-3567 0365-0340
DOI:	10.1080/03650340.2012.682221
Popis:	Potent bacterial strains effective against the whitefly, Bemisia tabaci, nymphs (second instar), were isolated from tomato cultivated fields at Fayoum governorate, Giza, Egypt. Of 72 isolates, 12 with the most morphologically distinct-looking bacterial colonies were selected and named A1, A2, A3, A6, A7, A9, A12, A13, A107, B37, B45 and B100. All isolates were preliminarily identified as members of the genus Bacillus based on morphological, physiological and biochemical characteristics. When tested for their pathogenicity against Bemisia tabaci, the 12 isolates revealed varying efficiencies with isolates A1 and A9 being superior, exhibiting maximum mortality of 92.2 and 90.8% on day 10, respectively. Isolate A7 recorded the lowest percentage at 18.3%. Further genetic characterization of the 12 isolates was performed using inter simple sequence repeat (ISSR), randomly amplified polymorphic DNA (RAPD) and 16S rDNA gene sequencing analysis. RAPD and ISSR results confirmed each other. The combined ISSR and RA...
Databáze:	OpenAIRE
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