Enhanced Production of Astaxanthin by Metabolically Engineered Non-mevalonate Pathway in Escherichia coli
| Autor: | Youn Su Cho, Tae Hyug Jeong, Han Kyu Lim, Seong-Seok Choi, Kun-Do Kim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Pigment Nutraceutical chemistry Biochemistry Astaxanthin visual_art Gene cluster medicine visual_art.visual_art_medium Non-mevalonate pathway Carotenoid Gene Escherichia coli Biotechnology |
| Zdroj: | Microbiology and Biotechnology Letters. 46:114-119 |
| ISSN: | 2234-7305 1598-642X |
| DOI: | 10.4014/mbl.1801.01007 |
| Popis: | Astaxanthin is one of the major carotenoids used in pigment has a great economical value in pharmaceutical markets, feeding, nutraceutical and food industries. This study was to increase the production of astaxanthin by co-expression with transformed Escherichia coli using six genes involved in the non-mevalonate pathway. Involved in the non-mevalonate biosynthetic pathway of the strain Kocuria gwangalliensis were cloned dxs, ispC, ispD, ispE, ispF, ispG, ispH and idi genes in order to increase astaxanthin production from the transformed E. coli. And co-expression with the genes to compared the amount of astaxanthin production. This engineered E. coli, containing both the non-mevalonate pathway gene and the astaxanthin biosynthesis gene cluster, produced astaxanthin at 1,100 μg/g DCW (dry cell weight), resulting in approximately three times the production of astaxanthin. |
| Databáze: | OpenAIRE |
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