Antibody Selection for the Abbott AxSYM Troponin I Assay
| Autor: | Cathy M. Forsythe, Don M. Laird, David H. Wilson, Anne L. Keller, Robert J. Herrmann, Tom T. Biegalski |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Clinical Chemistry. 45:2293-2294 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/45.12.2293 |
| Popis: | A recent report by Apple et al. (1) presented the performance characteristics of the AxSYM Troponin I (cTnI) assay, which included method comparison data with the Dade-Behring Stratus, Behring Opus, and Beckman Access cTnI assays. In commenting on the absolute differences in measured cTnI between methods (2- to 100-fold), the authors spoke of the need for unified rationale for antibody selection in assays for cTnI. Indeed, this has been a topic of substantial discussion in the recent literature in view of the large differences observed between cTnI methods [e.g., see Ref. (2)]. Most recently, Shi et al. (3) added to earlier results of Katrukha et al. (4) on cTnI degradation. In both reports, degradation of cTnI and differential epitope specificities between commercial methods were cited as significant contributors to between-method variation. Both studies also indicated that the C-terminal portion of the molecule is labile to proteolytic degradation, whereas the N-terminal half of the molecule is relatively stable. Katrukha … |
| Databáze: | OpenAIRE |
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