| Popis: |
Popularly referred to as lemon balm, Melissa officinalis L., has been used as a remedy for gastrointestinal disorders, respiratory and cardiovascular diseases, mental and central nervous system problems, various cancers, headache, nervousness and rheumatism. In this study, the phenolic profile, antioxidant potential, antibacterial activity, and enzyme activity of lemon balm grown in nature were determined and compared with in vitro-grown plants. Individual phenolic analysis with HPLC-DAD showed that the most prevalent phenol was rosmarinic acid in both extracts and naturally-grown plants had higher amount than in vitro-grown ones. Similarly, naturally-grown plants had considerably greater levels of total phenol-flavonoid, scavenging activity for free radicals (DPPH), and phenol synthesis related enzyme (PAL). Enzymatic antioxidant acticities (SOD and CAT) indicated that naturally grown plants had higher CAT activity and lower SOD activity. Noticeable result was obtained with antibacterial activity revealing moderate effect with in vitro-grown plants and no activity with naturally-grown ones. In general, these results showed that the M. officinalis grown in nature is exposed to more biotic and abiotic stress, and increases their phenolic content remarkably and consequently antioxidant capacity. |
