Reaction of human macrophages on protein corona covered TiO2 nanoparticles

Autor: Marcus Schäfer, Harald Fuchs, Klaus Langer, Yuliang Zhao, Cengiz Öztürk, Michael Mormann, Sylvia Wagner, Chunying Chen, Camila Figueiredo Borgognoni, Ying Qu, Kristina Riehemann
Rok vydání: 2015
Předmět:
Zdroj: Nanomedicine: Nanotechnology, Biology and Medicine. 11:275-282
ISSN: 1549-9634
Popis: The cytokine secretion of primary cells of human macrophages during the interaction of TiO 2 nanoparticles (with an average primary size of 100-120nm) is investigated down to concentration levels suggested to be relevant for in vivo conditions. We find that high TiO 2 concentrations induce the cytokines Interleukin IL-1β, IL-6, and IL-10 secretion, while at low concentrations only IL-6 secretion is observed. To obtain further evidence on in vivo conditions we investigated the development and structure of the protein corona of the nanoparticles. We demonstrated that the surface of TiO 2 particles attract preferably secondary modified proteins which then induce cytokine secretion of macrophages. Our results indicate that concentration of corona covered TiO 2 particles below 1μg/ml induce IL-6 secretion which is reported to be responsible for the development of autoimmune diseases as well as for the secretion of acute phase proteins. From the Clinical Editor This study investigates the effects of protein corona covered titanium dioxide nanoparticles on human macrophages, concluding that concentration of such particles below 1 μg/ml induces IL-6 secretion, which may be responsible for the development of autoimmune diseases as well as for the secretion of acute phase proteins. This finding has important implications on future applications of such nanoparticles.
Databáze: OpenAIRE
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