| Autor: |
Bui Van Le, K. Tran Thanh Van, Duong Tân Nhut |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie. 322:303-310 |
| ISSN: |
0764-4469 |
| DOI: |
10.1016/s0764-4469(99)80066-3 |
| Popis: |
Transverse thin cell layers (tTCLs) (0.2–0.4 mm thick) excised from the pseudo-bulblets of L. longiflorum in vitro were placed on the Murashige and Skoog medium supplemented with 3 % sucrose and a concentration of N-(2-chloro-4-pyridyl)-N′-phenylurea (forchlofenuron, CPPU) varying from 0.1 to 10 μM. Buds were obtained from the tTCL pseudo-bulblet expiants cultured in the presence of CPPU in the light but not in the dark. The number of buds formed per tTCL was the highest with 1 or 3 μM CPPU (16.8 ± 1.1 and 16.2 ± 1.6 buds per tTCL, respectively). Testing a combination of sucrose (from 1 to 12 %) and CPPU (from 1 to 10 μM) confirmed that the number of buds produced per tTCL was the highest when 1 or 3 % sucrose was combined with 1 or 3 μM of CPPU. The regenerated shoots were transferred to soil after rooting with 10 μM indole-3-byturic acid and normal plants were produced. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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