Effect of KCN and p-chloromercuribenzenesulfonic acid on the release of sucrose and 2-amino(1-14C)isobutyric acid by the seed coat of Pisum sativum

Autor:	Pieter Wolswinkel, Ankie Ammerlaan, Herbert Kuyvenhoven
Rok vydání:	1983
Předmět:	chemistry.chemical_classification Sucrose Physiology food and beverages Cell Biology Plant Science General Medicine Biology biology.organism_classification Apoplast Amino acid Pisum Isobutyric acid chemistry.chemical_compound chemistry Biochemistry Genetics Phloem Sugar Ovule
Zdroj:	Physiologia Plantarum. 59:375-386
ISSN:	1399-3054 0031-9317
Popis:	The process of sugar and amino acid release by the seed coat of Pisum sativum L. cv. Marzia was studied. Prior to measuring the release of solutes by the seed coat of developing ovules, the embryo was removed from each ovule studied. After this surgical treatment, each “empty” seed coat was filled with the appropriate solution (pH 5.5) with or without inhibitor. Both KCN and p-chloromercuribenzenesulfonic acid (PCMBS) strongly inhibited the release of sucrose and p-aminoisobutyric acid (AIB) by the seed-coat. These data support the view that phloem unloading is an energy-dependent process sensitive to the sulfhydryl group modifier PCMBS. In pulse-labelling experiments, addition of high concentrations of unlabelled sucrose (200 mM) and AIB (25 mM) to the solution filling the seed coat cavity did not diminish the release of labelled solutes by the unloading sites of the seed coat. This observation presents evidence against the view that phloem unloading into a strong sink is related to low sugar concentrations in the apoplast.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e40f898cc49f89b3e1cd59dfa07f9108 https://doi.org/10.1111/j.1399-3054.1983.tb04218.x Zobrazit plný text záznamu Plný text